Whole-Genome Sequence Insight into the Plant-Growth-Promoting Bacterium Priestia filamentosa Strain AZC66 Obtained from Zygophyllum coccineum Rhizosphere

This study aimed to isolate, screen the plant-growth-enhancing features, and explore whole-genome sequence of AZC66 isolated from rhizosphere Zygophyllum coccineum determine its biostimulating effects on growth cowpea under greenhouse conditions. Salkowski reagent was used measure AZC66’s indole acetic acid production. inorganic phosphate solubility Pikovskaya agar evaluated using tricalcium phosphate. The results indicated ability fix nitrogen, produce IAA (66.33 ± 0.44 μg mL−1), solubilize phosphate, exhibit activity ACC deaminase (278.40 21 mol -ketobutyrate mg−1 h−1). Cowpea’s root shoot dry weights were also significantly increased after in vitro inoculation with AZC66. identity confirmed as Priestia filamentosa, 4840 genes predicted genome. gene sequences compared against Kyoto Encyclopedia Genes Genomes (KEGG) database, showed that top three pathways wherein maximum number are involved signaling cellular processes, genetic information processing, carbohydrate metabolism. genome sequencing strain revealed a implicated plant biostimulation activities such nitrogen fixation (nifU), phytohormone synthesis (trpAB genes), solubilization (PhbCEF, pstABCS, phoU), siderophore formation (FbpA, feoAB, fetB). contained numerous metabolism, regulation, nitrate reduction pathway. phenazine biosynthetic demonstrated biocontrol soil survival properties. trehalose may help plants resist osmotic salt stress. discovery glycine betaine, cold shock, heat shock protein could withstand harsh might be create robust, sustainable biological fertilizers for future agricultural use Saudi Arabia. Furthermore, adaptable metabolic serve basis potential biotechnological applications agriculture industry.